A Disposable, High-Yield Cell Culture System
Introducing FibraStage, a disposable cell culture
system that produces high product yields while decreasing labor
demands over traditional cell culture devices.
FibraStage is an inexpensive and easy-to-use
system, capable of producing mg quantities of protein, virus or
biomass from a wide variety of anchorage-dependent or suspension
cultures. Depending on your cell line and desired end product, up to
four FibraStage bottles can produce yields of 2.4 x 10 10 with minimal effort. A single FibraStage bottle can produce a
high-cell density of 6 x 10 9. Here's how:
Disposable FibraStage bottles are
pre-sterilized and pre-loaded with 10 grams of FibraCel disks and
each bottle can hold up to 500 mL of liquid. FibraCel disks are a
unique solid-support matrix for producing high-density cultures.
Cells grow in, on and between the porous, multi-layer disks, which
provide an extremely high surface area for cell growth, equivalent
to 12,000 cm2. To obtain an equivalent surface area of a single
FibraStage bottle, one would need to maintain numerous spinners,
dozens of roller bottles and hundreds of T-flasks.
- Maintaining growth in one FibraStage bottle vs.
dozens or even hundreds of T-flasks, spinners, bottles or bags is
far more efficient in terms of time and labor
Furthermore, cells remain entrapped in the
disk bed during the entire run, keeping the medium virtually cell
free. This further reduces labor by:
- simplifying media replacement and simplifying
The system also eliminates:
- autoclaving, as bottles are
pre-sterilized and ready to go
- cleaning - as without the need
to autoclave, there is no need to validate the cleaning process
- lengthy learning curves, as
the system is plug-and-play simple
- costly start-up, as there is
no need for expensive designs, nor piped-in steam, water lines or
other utilities; and
- cross-contamination between batches - simply dispose of the bottle when done.
Operation is Simple
Gas exchange and media mixing are accomplished
simply and efficiently by placing up to four FibraStage bottles in a
FibraStage device, which slowly raises and lowers the media level in
the bottles. This up-down movement collapses or expands bellows at
the base of each bottle. In the "up" position, the media is forced
through the disk bed, facilitating nutrient feeding and removal of
waste from the cells. During the "down" cycle, cells are exposed to
air and gasses. This process enables efficient oxygen transfer to
the cells, while providing the cells with nutrients. A controller
allows setting variable speeds for the up and down motions, and
variable "hold times" during which the cells are exposed to media or
gas. A 0.22 ¥ìm PTFE filter is supplied in the bottle cap for
- Each FibraStage system holds up to four
FibraStage bottles, making this an ideal screening device to
test varying media formulations or cell lines. (The new
continuous culture system accommodates three bottle.)
- The compact system fits into most standard CO2
incubators*. The controller is magnetized so it can be
conveniently stored on the outside wall of the incubator.
Incubators are available from NBS or can be user-supplied.
New Continuous Cultue
- A new continuous culture system with
pumps is available for constant exchange of nutrients,
eliminating the labor of media exchange and avoiding possible
shock to cells during culture.
- Bottles are equipped with additional inlet
& outlet lines for re-circulating media to external 250
mL, 500 mL or 2L storage vessels.
- Includes console, controller, 3-pump module
with two peristaltic pump heads and tubing. Shown with
optional third pump head & tubing.
- Programmable and On/Off pumping control
eliminates the requirement to calibrate the flow rate and
simplifies the setting of re-circulation rate for each