Spinner Basket with Fibra-Ce Support
Producing milligram to gram quantities of secreted protein or high
virus titer from suspension, insect or recombinant anchorage-dependent
cells (including primary cells)
- Immobilizes cells on Fibra-Cel disks while secreted proteins
are easily harvested with the medium
- Cultivates hybridomas, recombinant adherent cell lines and
insect cells all in one system.
- Eliminates the need to pass cells - saving time and labor.
- Longer culture times - Up to 30 days continuously.
- Simplifies harvesting and purification - No need to separate
- Acheives higher cell densities than conventional spinners: >
107 cells per cm3 of bed volume
- Low pressure drop across Fibra-Cel bed allows for high mass
transfer of nutrients and oxygen at high cell densities
- Cells can be grown with many types of serum-free media
- Able to grow primary cells
- Provides maximum 12,000 cm2 of surface area; equivalent
to 160 T-flasks or 14 roller bottles
- Entrapped cells protected from impeller shear and gas bubbles
- Reduced lag time: adherent cells attach more quickly compared
to conventional microcarriers
- No animal-derived compounds
- Will not leech pyrogens nor bind product
- Low disposable cost per run
- Can be validated as a cGMP process in production scale with
- Converts to dedicated mini bioreactor with optional headplate
and sensors for pH and DO
Fibra-Cel Increases Yields up to 10-Fold Over Microcarriers
Increased Productivity for a multitude of Cell Lines
If you're looking for increases in protein yields with big savings
in time and labor, you'll be excited about the Spinner Basket, a low
cost development tool designed for high density cell culture. With
Fibra-Cel, a unique support matrix, this spinner can produce record
yields of secreted proteins with less time and effort than ever before.
Fibra-Cel disks are a major improvement over microcarriers, and can
be used with all types of mammalian cells including suspension cultures.
Advantages of Cell Immobilization
The Spinner Basket combines the advantages of immobilized cell technology
with the simplicity of the Spinner Flask. Conventional Spinners are
unable to retain cells during exchange of media. In addition, cell
cartridges and other immobilization devices have been inherently expensive
and complex to operate and control. But with the use of Fibra-Cel,
the Spinner Basket now brings a high level of efficiency to the production
of secreted proteins while lowering the associated disposable costs.
Longer Cultivation Times Now Possible
The use of cell immobilization in the Sinner Basket enables cells
to be cultivated for 30 days and more in a shear-free environment.
Suspension hybridomas can now be cultured for long periods as they
continue to produce their secreted antibody without the need for passing
the cells each time media is exchanged. As a result, the Spinner Basket
is a very productive tool for producing diagnostic antibodies while
saving labor and time. Anchorage-dependent cell lines strongly attach
to the polyester fibers where they can be continuously cultured in
serum-free media as they secrete the desired recombinant protein.
Media optimization studies can now be performed with less effort,
more confidence and lower costs.
Fibra-Cel (A new support
system for mammalian cells)
The use of Fibra-Cel in the Spinner Basket is the key ingredient for
successful cell entrapment, growth, and product yield. Fibra-Cel's
high surface area to volume ratio and exceptionally low pressure drop
make it unique when compared to other known solid support growth matrices.
It is a disk wafer composed of two pure materials; polyester and polypropylene.
No other compounds are incorporated. The cells grow three-dimensionally
between or attached to the 15 micron polyester fibers while polypropylene
provides a support function only. Adherent cells attach to the polyester
fibers while suspension cells are entrapped between the fibers. Fibra-Cel
disks are electrostatically charged, similar to plastic tissue culture
ware, strongly binding suspension cells to the fibers.
Fibra-Cel provides a maximum of 12,000 cm2 of surface area
which is equivalent to 160 T-flasks or 14 roller bottles. When packed
inside the Spinner Basket it only occupies 10% of the bed space, allowing
for 90% void volume. This enables high cross-sectional liquid flow
rates through the bed producing efficient mass transfer of nutrients
and oxygen at high cell densities.
The cell immobilization technology employed in the Spinner Basket
has been successfully used in larger production scale bioreactors.
The low pressure drop across the disk bed at high cell densities (108
per cm3) differentiates Fibra-Cel from other traditional
growth matrices. High mass transfer of nutrients and oxygen are continuously
maintained to support high producing viable cultures. Many biopharmaceutical
companies are using Fibra-Cel in processes that will be validated
under cGMP conditions.