Bioreactor & Fermentor
Bioreactor & Fermentor
Spin filter
Spin filters are retention devices designed to keep the cells inside the vessel in perfusion cultivation.
Combined for example with low-shear marine impellers they can be used for suspension cells and cells growing on microcarriers.
The spin filter consists of a screened cage with very small pore size that keeps cells isolated outside the cage.
The cage rotates, attached to the concentric impeller shaft, while inside of it a dip tube continuously withdraws the filtered, cell-free culture broth.
For perfusion culture, an addition tube outside the cage provides a steady supply of fresh medium so that the working volume remains at a constant level.
This mechanism is ideal for production of secreted proteins: It keeps the harvested medium free of cells, which simplifies downstream purification.
Over time, however, the spin filter screen material will tend to clog and require replacement, thus limiting cultivation time.
Combined for example with low-shear marine impellers they can be used for suspension cells and cells growing on microcarriers.
The spin filter consists of a screened cage with very small pore size that keeps cells isolated outside the cage.
The cage rotates, attached to the concentric impeller shaft, while inside of it a dip tube continuously withdraws the filtered, cell-free culture broth.
For perfusion culture, an addition tube outside the cage provides a steady supply of fresh medium so that the working volume remains at a constant level.
This mechanism is ideal for production of secreted proteins: It keeps the harvested medium free of cells, which simplifies downstream purification.
Over time, however, the spin filter screen material will tend to clog and require replacement, thus limiting cultivation time.
